Imaging collective cell migration and hair cell regeneration in the sensory lateral line

M. Venero Galanternik, J. Navajas Acedo, A. Romero-Carvajal, T. Piotrowski

Research output: Research - peer-reviewArticle

Abstract

The accessibility of the lateral line system and its amenability to long-term in vivo imaging transformed the developing lateral line into a powerful model system to study fundamental morphogenetic events, such as guided migration, proliferation, cell shape changes, organ formation, organ deposition, cell specification and differentiation. In addition, the lateral line is not only amenable to live imaging during migration stages but also during postembryonic events such as sensory organ tissue homeostasis and regeneration. The robust regenerative capabilities of the mature, mechanosensory lateral line hair cells, which are homologous to inner ear hair cells and the ease with which they can be imaged, have brought zebrafish into the spotlight as a model to develop tools to treat human deafness. In this chapter, we describe protocols for long-term in vivo confocal imaging of the developing and regenerating lateral line.

LanguageEnglish (US)
JournalMethods in Cell Biology
DOIs
StateAccepted/In press - 2016

Fingerprint

Inner Auditory Hair Cells
Lateral Line System
Cell Shape
Deafness
Zebrafish
Inner Ear
Cell Movement
Regeneration
Cell Differentiation
Homeostasis

Keywords

  • Collective migration
  • Confocal imaging
  • Hair cells
  • Primordium
  • Regeneration
  • Sensory system
  • Spatial analysis
  • Time lapse
  • Transgenic lines
  • Zebrafish embryo

ASJC Scopus subject areas

  • Cell Biology

Cite this

Venero Galanternik, M., Navajas Acedo, J., Romero-Carvajal, A., & Piotrowski, T. (2016). Imaging collective cell migration and hair cell regeneration in the sensory lateral line. Methods in Cell Biology. DOI: 10.1016/bs.mcb.2016.01.004

Imaging collective cell migration and hair cell regeneration in the sensory lateral line. / Venero Galanternik, M.; Navajas Acedo, J.; Romero-Carvajal, A.; Piotrowski, T.

In: Methods in Cell Biology, 2016.

Research output: Research - peer-reviewArticle

Venero Galanternik, M, Navajas Acedo, J, Romero-Carvajal, A & Piotrowski, T 2016, 'Imaging collective cell migration and hair cell regeneration in the sensory lateral line' Methods in Cell Biology. DOI: 10.1016/bs.mcb.2016.01.004
Venero Galanternik M, Navajas Acedo J, Romero-Carvajal A, Piotrowski T. Imaging collective cell migration and hair cell regeneration in the sensory lateral line. Methods in Cell Biology. 2016. Available from, DOI: 10.1016/bs.mcb.2016.01.004
Venero Galanternik, M. ; Navajas Acedo, J. ; Romero-Carvajal, A. ; Piotrowski, T./ Imaging collective cell migration and hair cell regeneration in the sensory lateral line. In: Methods in Cell Biology. 2016
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